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episheartm probe sonicator  (Active Motif)


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    Structured Review

    Active Motif episheartm probe sonicator
    Episheartm Probe Sonicator, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/episheartm+probe+sonicator/episheartm+probe+sonicator/10__1016_slash_j__jddst__2025__106607-72-10-13
    Average 90 stars, based on 1 article reviews
    episheartm probe sonicator - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Lysis:

    Article Title: Reversible switching and stability of the epigenetic memory system in bacteria.
    Article Snippet: .. Cell lysis by sonication was performed using the EpiShearTM Probe Sonicator (Active Motif Europe, Waterloo, Belgium). ..

    Article Title: The MYC–NFATC2 axis maintains the cell cycle and mitochondrial function in acute myeloid leukaemia cells
    Article Snippet: .. Cells were fixed using formaldehyde (1% V/V in H 2 O) for 10 min at RT before supplementation with 125 m m glycine solution (Merck) and agitation for 5 min. Cytosolic lysate was generated from fixed cells using cytoplasmic lysis buffer (10 m m Tris pH 8.0/10 m m NaCl/0.4% (V/V) IGEPAL CA‐630/1× protease inhibitor cocktail/10 μ m DIFP; Merck) and removed, before generation of nuclear lysate using nuclear lysis buffer (50 m m Tris pH 8.0/EDTA 50 m m /SDS 0.8% (W/V)/1× protease inhibitor cocktail/10 μ m DIFP; Merck), which was then sonicated using an EpiShearTM Probe Sonicator (Active Motif, Waterloo, Belgium), with 18 bursts at 30% amplitude for 30 s per burst. .. Sheared lysates were pre‐cleared using 2 μg rabbit IgG antibody (Thermo Fisher Scientific) per sample for 1 h at 4 °C, before removing IgG‐bound lysate by incubating with protein G magnetic Dynabeads ® (Thermo Fisher Scientific) for 1 h and then removal by incubation with a magnetic stand.

    Sonication:

    Article Title: Reversible switching and stability of the epigenetic memory system in bacteria.
    Article Snippet: .. Cell lysis by sonication was performed using the EpiShearTM Probe Sonicator (Active Motif Europe, Waterloo, Belgium). ..

    Article Title: Mutual Amplification of GLI2 /Hedgehog and Transcription Factor JUN / AP ‐1 Signaling in Fibroblasts in Systemic Sclerosis: Potential Implications for Combined Therapies
    Article Snippet: ChIP assays 102 were performed using the ChIP-IT High Sensitivity Kit (Active Motif, Eching, Germany). .. After 103 TGFβ stimulation, cells were fixated according to the manufacturer’s instructions and 104 chromatin was sonicated using an EpiShearTM Probe Sonicator (Active Motif, Eching, 105 Germany). .. Sonicated chromatin was incubated with antibodies targeting cJUN (ab31419, 106 Abcam, Cambridge, United Kingdom) and GLI2 (sc-271786 X, Santa Cruz Biotechnology, 107 Heidelberg, Germany) or rabbit IgG antibody (#NI01; Millipore, Darmstadt, Germany).

    Article Title: The MYC–NFATC2 axis maintains the cell cycle and mitochondrial function in acute myeloid leukaemia cells
    Article Snippet: .. Cells were fixed using formaldehyde (1% V/V in H 2 O) for 10 min at RT before supplementation with 125 m m glycine solution (Merck) and agitation for 5 min. Cytosolic lysate was generated from fixed cells using cytoplasmic lysis buffer (10 m m Tris pH 8.0/10 m m NaCl/0.4% (V/V) IGEPAL CA‐630/1× protease inhibitor cocktail/10 μ m DIFP; Merck) and removed, before generation of nuclear lysate using nuclear lysis buffer (50 m m Tris pH 8.0/EDTA 50 m m /SDS 0.8% (W/V)/1× protease inhibitor cocktail/10 μ m DIFP; Merck), which was then sonicated using an EpiShearTM Probe Sonicator (Active Motif, Waterloo, Belgium), with 18 bursts at 30% amplitude for 30 s per burst. .. Sheared lysates were pre‐cleared using 2 μg rabbit IgG antibody (Thermo Fisher Scientific) per sample for 1 h at 4 °C, before removing IgG‐bound lysate by incubating with protein G magnetic Dynabeads ® (Thermo Fisher Scientific) for 1 h and then removal by incubation with a magnetic stand.

    Centrifugation:

    Article Title: MUC1 promotes lymph node metastasis in esophageal squamous cell carcinoma by downregulating DNAJB6 expression.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81902418; Natural Science Foundation of Shandong Province, Grant/Award Number: ZR2020MH244 Abstract Background: Aberrant expression of MUC1 correlates with the progression of esophageal squamous cell carcinoma (ESCC), this study aimed to explore the effect of targeting MUC1 by Go-203 on malignant behavior of ESCC and the underlying mechanism.. Methods and Results: IHC was used to examine the expression of MUC1 and DNAJB6 in ESCC samples. qRT–PCR and western blotting were used to examine the expression of MUC1 and DNAJB6 in ESCC cell lines.. CCK8, wound healing, and transwell assays were used to determine the effect of regulating MUC1/DNAJB6 on the proliferation, migration, and invasion of ESCC cells.

    Isolation:

    Article Title: Locus-Specific and Stable DNA Demethylation at the H19 / IGF2 ICR1 by Epigenome Editing Using a dCas9-SunTag System and the Catalytic Domain of TET1.
    Article Snippet: CTCF-ChIP was performed using the Magna ChIPTM HiSens kit (Catalog No. 17- 10460, Merck KGaA, Darmstadt, Germany), according to the manufacturer’s protocol. .. Isolated crosslinked chromatin from 4 million cells was sheared using the EpiShearTM probe sonicator (Active Motif, Waterloo, Belgium) with the 2 mm tip (40% amplitude, 12 × 20 s pulse, and 30 s pause between each pulse). .. For each ChIP reaction, 50 or 100 μL of crosslinked and sonicated chromatin and 3 μL of αCTCF antibody (CTCF rabbit AB, Cell Signaling Technology Europe, Frankfurt am Main, Germany, catalog #2899S, Lot: 2) were used.

    Generated:

    Article Title: The MYC–NFATC2 axis maintains the cell cycle and mitochondrial function in acute myeloid leukaemia cells
    Article Snippet: .. Cells were fixed using formaldehyde (1% V/V in H 2 O) for 10 min at RT before supplementation with 125 m m glycine solution (Merck) and agitation for 5 min. Cytosolic lysate was generated from fixed cells using cytoplasmic lysis buffer (10 m m Tris pH 8.0/10 m m NaCl/0.4% (V/V) IGEPAL CA‐630/1× protease inhibitor cocktail/10 μ m DIFP; Merck) and removed, before generation of nuclear lysate using nuclear lysis buffer (50 m m Tris pH 8.0/EDTA 50 m m /SDS 0.8% (W/V)/1× protease inhibitor cocktail/10 μ m DIFP; Merck), which was then sonicated using an EpiShearTM Probe Sonicator (Active Motif, Waterloo, Belgium), with 18 bursts at 30% amplitude for 30 s per burst. .. Sheared lysates were pre‐cleared using 2 μg rabbit IgG antibody (Thermo Fisher Scientific) per sample for 1 h at 4 °C, before removing IgG‐bound lysate by incubating with protein G magnetic Dynabeads ® (Thermo Fisher Scientific) for 1 h and then removal by incubation with a magnetic stand.

    Protease Inhibitor:

    Article Title: The MYC–NFATC2 axis maintains the cell cycle and mitochondrial function in acute myeloid leukaemia cells
    Article Snippet: .. Cells were fixed using formaldehyde (1% V/V in H 2 O) for 10 min at RT before supplementation with 125 m m glycine solution (Merck) and agitation for 5 min. Cytosolic lysate was generated from fixed cells using cytoplasmic lysis buffer (10 m m Tris pH 8.0/10 m m NaCl/0.4% (V/V) IGEPAL CA‐630/1× protease inhibitor cocktail/10 μ m DIFP; Merck) and removed, before generation of nuclear lysate using nuclear lysis buffer (50 m m Tris pH 8.0/EDTA 50 m m /SDS 0.8% (W/V)/1× protease inhibitor cocktail/10 μ m DIFP; Merck), which was then sonicated using an EpiShearTM Probe Sonicator (Active Motif, Waterloo, Belgium), with 18 bursts at 30% amplitude for 30 s per burst. .. Sheared lysates were pre‐cleared using 2 μg rabbit IgG antibody (Thermo Fisher Scientific) per sample for 1 h at 4 °C, before removing IgG‐bound lysate by incubating with protein G magnetic Dynabeads ® (Thermo Fisher Scientific) for 1 h and then removal by incubation with a magnetic stand.



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